What is GHK-Cu?

GHK-Cu (glycyl-L-histidyl-L-lysine copper(II)) is a low-molecular-weight peptide-metal complex consisting of a GHK tripeptide (Gly-His-Lys) coordinated with a copper(II) ion. The compound occurs naturally in mammalian bodies, where it has been identified in plasma, saliva and extracellular matrix. Its structure and physicochemical properties were characterized in detail in the second half of the 20th century in the context of research on metal-binding peptides and their role in regulating cellular processes. Due to the stability of the complex and its good solubility in aqueous media, GHK-Cu has become a useful model in studies of peptide signaling and metal ion homeostasis.

In experimental studies, GHK-Cu has been used to analyze mechanisms related to the regulation of gene expression, extracellular matrix metabolism and cellular response to oxidative stress. Its effects on modulation of metalloproteinase enzyme activity, expression of growth factors and structural proteins, as well as on processes related to cell proliferation and differentiation in in vitro and in vivo models were described. Attention was also paid to its ability to participate in redox reactions and indirect effects on pro- and antioxidant balance under experimental conditions.

Due to its unambiguous chemical structure, high reproducibility of observed biological effects and natural occurrence in mammalian organisms, GHK-Cu is used as a research tool in molecular biology, cell biology and biochemistry. It is also sometimes used in the scientific literature as a reference peptide in studies of extracellular matrix remodeling, regulation of gene expression, and validation of analytical methods used in the analysis of short peptides and peptide-metal complexes. The preparation is intended for research work only.

Technical parameters of GHK-Cu peptide

Name of substance: GHK-Cu (glycyl-L-histidyl-L-lysine copper(II))
Type of relationship: Peptide-metal complex - GHK tripeptide coordinated with copper(II) ion
Amino acid sequence: Gly-His-Lys (GHK) + Cu²⁺.
Summary formula: C₁₄H₂₄N₆O₄Cu.
Molecular weight: ~401.9 g/mol
CAS number: 89030-95-5
Physical form: Blue or blue-green powder after freeze-drying
Solubility: soluble in aqueous solutions

Product quality (GHK-Cu 50 mg + bacteriostatic water)

Our product has been tested by a certified Polish laboratory. The results are available in the product gallery. The product is endotoxin free.

Package contents

Sterile vial of GHK-Cu acetate 50mg powder (peptide stabilizer - mannitol)
Sterile vial of 3 ml bacteriostatic water (0.9% benzyl alcohol)

What does the product package (GHK-Cu 50 mg + bacteriostatic water) contain?

The ordered product will come packaged in a sturdy tube resistant to transport damage, and inside it you will find:

Sterile vial of GHK-Cu acetate 50mg powder (peptide stabilizer - mannitol)
Sterile vial of 3 ml bacteriostatic water (0.9% benzyl alcohol)

The product has an expiration date of a minimum of one year and a maximum of two years from the date of shipment - depending on the batch.

The safety data sheet (SDS) for this product can be downloaded here

How to prepare the solution for the laboratory test?

Remove the caps from both vials.
Apply spirit to a cotton swab and disinfect the rubber bands in both vials.
Using an insulin syringe, pour 3ml of bacteriostatic water into the powder vial (along the wall of the vessel)
Roll the vial in your hands or spin the vial making a vortex until the solution dissolves. This activity can be done vigorously, it is important not to foam the solution with strong shaking.
The solution prepared in this way gives us a concentration of 2.5 mg of GHK-Cu peptide for every 0.15 ml of solution, which should be stored in the refrigerator from now on.

Storage and transportation

In dry form, the peptide should be stored at room temperature away from direct sunlight. Even longer transportation by courier will not affect the stability and quality of the product.

In wet form (after preparing the solution), the peptide should be in the refrigerator all the time and used up within 30 days.

Disclaimer

The product is not a drug, food product or dietary supplement. The product is classified as a chemical reagent/reference material authorized in the European Union under the European REACH Act, and can only be used for laboratory scientific research. Educational materials about GHK-Cu substance posted on our site are written on the basis of scientific research on the substance. These studies were performed by qualified scientific centers around the world and are not product specific.